Regulatory

Part:BBa_K2924016:Design

Designed by: Mirko Kraus   Group: iGEM19_Duesseldorf   (2019-10-14)


Promoter fliC from the Escherichia coli genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

This promoter is sensitive to short chain fatty acids and was proven in literature to regulate chemotaxis.

Source

The DNA sequence was take from Tobe et al.1 and synthesized by IDT.

References

1: Toru Tobe,* Noriko Nakanishi, and Nakaba Sugimoto “Activation of Motility by Sensing Short-Chain Fatty Acids via Two Steps in a Flagellar Gene Regulatory Cascade in Enterohemorrhagic Escherichia coli” INFECTION AND IMMUNITY, Mar. 2011, p. 1016–1024